Specifications
- Purity
- for microscopy
- Appearance
- Dark red to brown liquid
Properties
- Refractive Index
- n20/D 1.334 - 1.336
- Density
- 1.003 - 1.007 g/mL at 20 °C
Documentation
- Safety Data Sheet (SDS)
- CDX G0065 MSDS.pdf
Available from stock
Gram’s iodine solution
- SKU
- G0065
Category: Non Fluorescent Detection & Chromogene Detection
- 7553-56-2 (component)
- CAS-Number
- n/a
- Molecular Formula
- n/a
- Molecular Weight
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
- Shipping
- AMBIENT
- Short Term Storage
- RT
- Long Term Storage
- RT
- Handling Advice
- Protect from light and moisture.
- Use / Stability
- This product should not be used if colour has changed or if there are
- Hazard statements
- H402
- Precautionary statements
- P273, P501
- Transportation
- Not dangerous goods
- Description
- Gram?s iodine solution acts as a mordant for Gram staining technique. It forms an insoluble crystal violet-iodine complex, which imparts purple color to the cells. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
- References
- (1) E. Adams, Stain Technol. 50, 227 (1975) , (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005)
Gram?s iodine solution acts as a mordant for Gram staining technique. It forms an insoluble crystal violet-iodine complex, which imparts purple color to the cells. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Additional information
| Purity | for microscopy |
|---|---|
| Appearance | Dark red to brown liquid |
| CAS-Number | 7553-56-2 (component) |
| Molecular Formula | n/a |
| Molecular Weight | n/a |
| Refractive Index | n20/D 1.334 – 1.336 |
| Density | 1.003 – 1.007 g/mL at 20 °C |
| Shipping | AMBIENT |
| Short Term Storage | RT |
| Long Term Storage | RT |
| Handling Advice | Protect from light and moisture. |
| Use / Stability | This product should not be used if colour has changed or if there are |
| Hazard statements | H402 |
| Precautionary statements | P273, P501 |
| Transportation | Not dangerous goods |
| References | (1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) |
| Quantity | 250 ml, 2.5 l, Bulk |
