Specifications
- Purity
- for microscopy
- Appearance
- Dark red to brown liquid
Properties
- Refractive Index
- n20/D 1.334 - 1.336
- Density
- 1.003 - 1.007 g/mL at 20 °C
Downloads
- Safety Data Sheet
- CDX G0065 MSDS.pdf
Available from stock
Category: Non Fluorescent Detection & Chromogene Detection
Gram?s iodine solution acts as a mordant for Gram staining technique. It forms an insoluble crystal violet-iodine complex, which imparts purple color to the cells. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Purity | for microscopy |
---|---|
Appearance | Dark red to brown liquid |
CAS-Number | 7553-56-2 (component) |
Molecular Formula | n/a |
Molecular Weight | n/a |
Refractive Index | n20/D 1.334 – 1.336 |
Density | 1.003 – 1.007 g/mL at 20 °C |
Shipping | AMBIENT |
Short Term Storage | RT |
Long Term Storage | RT |
Handling Advice | Protect from light and moisture. |
Use / Stability | This product should not be used if colour has changed or if there are |
Hazard statements | H402 |
Precautionary statements | P273, P501 |
Transportation | Not dangerous goods |
References | (1) E. Adams, Stain Technol. 50, 227 (1975), (2) R. Coico, et al., Curr. Protoc. Microbiol. Appendix 3-3C (2005) |
Quantity | 250 ml, 2.5 l, Bulk |