Molecular Biology & Analytical Reagents
HPLC is widely employed for detecting trace components, and enhancing sensitivity and selectivity is achieved through derivatization with labeling reagents. Numerous labeling reagents are available, including a potent alkylation agent for direct methylation and trifluoroethylation. Additionally, a highly sensitive fluorescent stain facilitates the detection of total proteins in polyacrylamide gel electrophoresis, while a specialized label serves as a sensitive marker for time-resolved fluoroimmunoassay with distinct characteristics such as a large Stoke’s shift and narrow emission bands.
