Gram Staining is a differential staining technique most widely applied in all microbiology laboratories. It is one of the most important criteria in any identification scheme for all types of bacterial isolates. Different mechanisms have been proposed to explain the Gram reaction. There are many physiological differences between Gram-positive and Gram-negative cell walls. Ever since Christian Gram has discovered Gram staining, this process has been extensively investigated and redefined. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.

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