PH Indicators / Probes

Intracellular pH is generally between ~6.8 and 7.4 in the cytosol and ~4.5 and 6.0 in acidic organelles such as lysosomes. Unlike intracellular free Ca2+ concentrations, which can rapidly change by perhaps 100-fold, pH inside a cell varies by only fractions of a pH unit and such changes may occur quite slowly. Fluorescent dyes provide the increased sensitivity required for optical pH measurements inside live cells and to sense pH changes within physiological ranges.
Chemodex offers a range of indicators for tracking intracellular pH in the cytosol or in particular organelles.

BCECF-AM

BCECF-AM
CDX-C0003                                                                       1 mg | 5 mg
BCECF-AM is a widely-used fluorescent indicator for measuring
intracellular pH. This ester can also be used to investigate intracellular changes in other ions, including potassium.
Spectral Properties: λex 490 nm; λem 535 nm.

PRODUCT NAME SUK DESCRIPTION
Fluorescein octadecyl ester F0074 Lipophilic fluorescent pH indicator.
HPTS H0034 Fluorescent pH indicator for physiological range.
Neutral Red 5 N0249 Cellular pH indicator for histology stainings, changing from Red to Yellow between pH 6.8 and 8.0.
Phenol red sodium salt P0026 Water soluble pH indicator used in the 6.8 (Yellow) to 8.2 (Red) range
Xylenol Blue X0006 Acid-base pH indicator dye with pH ranges of pH 1.2 (Red) – pH 2.8 (Yellow), and pH 8.0 (Yellow) – pH 9.6 (Blue).

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