PH Indicators / Probes

Intracellular pH is generally between ~6.8 and 7.4 in the cytosol and ~4.5 and 6.0 in acidic organelles such as lysosomes. Unlike intracellular free Ca2+ concentrations, which can rapidly change by perhaps 100-fold, pH inside a cell varies by only fractions of a pH unit and such changes may occur quite slowly. Fluorescent dyes provide the increased sensitivity required for optical pH measurements inside live cells and to sense pH changes within physiological ranges.
Chemodex offers a range of indicators for tracking intracellular pH in the cytosol or in particular organelles.


CDX-C0003                                                                       1 mg | 5 mg
BCECF-AM is a widely-used fluorescent indicator for measuring
intracellular pH. This ester can also be used to investigate intracellular changes in other ions, including potassium.
Spectral Properties: λex 490 nm; λem 535 nm.