Calcium Indicators & Chelators
Calcium (Ca2+) acts as a universal second messenger in nearly every aspect of cellular life. Ca2+-mediated signal transduction has specific roles in exocytosis, motility, apoptosis and transcription. Numerous functions of all types of cells are regulated by Ca2+, thus its measurement is critical for various biological investigations. Ca2+ indicators and chelators are fluorescent probes that show spectral responses upon binding Ca2+. They have enabled researchers to investigate changes in intracellular and extracellular Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence
spectroscopy and fluorescence microplate readers.
Rhod-2-AM
CDX-A0072 1 mg
Long-wavelength cell-permeant Ca2+ indicator allowing noninvasive measurement of calcium ions in live cells. Valuable alternative in cells and tissues that have high levels of autofluorescence. Rhod-2 has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, whose excitation and emission maxima make it suitable for use with argon and krypton laser. Spectral Properties: λex 550 nm, λem 578 nm in methanol.
